This message has been posted by: 612 right <i.hinners at REMOVE-THIS-TO-SENDicrf.icnet.uk>
Hello Dave,
I think it almost purely depends on the protein and its modification.
Glycosylation often results in a smear. I am working with a protein which migrates
faster after myristoylation, but other proteins would migrate slower. I believe
phosphorylation usually descreases mobility.... and so on....
So there is no strict rule or even a rule to calculate the mobility,
hope this helps, Ina
"D.D. Morgan" wrote:
> How does modification of a protein alter its apparent mwt on SDS-PAGE ?
>> Is it purely the increase in mwt due to the added residues or do they have
> an effect on the overall charge of the protein altering its mobility ?
>> Any answers gratefully recieved and are there any basic references for a
> "DNA man" to check out
>> Thanks
>> Dave Morgan
>> --
>> *******************************************************************************
> * Dave Morgan | **. .***. .***. .***. .** *
> * Dept of Human Genetics | | | * * | | | * | | | * * | | | * | | *
> * University of Newcastle | | | | * * | | | * * | | | * * | | | * * | *
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--
Ina Hinners
ICRF
Secretory Pathways Laboratory
44 Lincolns Inn Fields
WC2A 3PX London
UK
email: I.Hinners at icrf.icnet.uk
