Ben Gantner wrote:
>> Hi all,
>> Does anyone have protocols or good web sites that discuss methods for
> improving resolution of SDS-PAGE? I'm trying to get separation of a
> fairly complex mixture, and I'm not getting particularly good
> resolution.
>>Without more detail on your particular application its hard to help you
.
Having run hundreds of SDS-Page on plant proteins some years ago, here
are a few words of advise:
Use a good cooled set up like biorad protean II
Run gradient gels.
I suspect that the precast gels they sell now days may be a good
investment, but never had those.
Use fresh acrylamide, from good source, filtered through .44 um filter
Sample preparation is everything. Use proteinase inhibitors. Work
fast , keep samples cold, spin the samples hard to remove
particulates.
