Hi,
I usually fix the gel in 10%(v/v) MeOH / 10%(v/v) acetic acid then stain
with 0.25%(w/v) Coomassie Brilliant Blue R-250 in 50%(v/v) MeOH / 10%
acetic acid. The fixing step is probably not really required as the
staining solution should fix the gel. I then destain in 45%(v/v) MeOH /
10%(v/v) acetic acid. Seems to work well...
**************************************************
Matt Hicks
Centre for Biomolecular Design & Drug Development
School of Biological Sciences
University of Sussex
Falmer
BN1 9QG
UK
Tel: +44(0)1273 678923
Fax: +44(0)1273 678433
email: matth at biols.sussex.ac.uk
**************************************************
On Mon, 13 Mar 2000, David Coulson wrote:
> I was wondering if anybody could help me with a "recipe" for
> making up Collodial Blue (Comassie G-250) solution for
> staining SDS-PAGE gels. Do you just make it up in water?,
> and if so what sort of concentration?
>> Any help would be appreciated
>>> * Sent from AltaVista http://www.altavista.com Where you can also find related Web Pages, Images, Audios, Videos, News, and Shopping. Smart is Beautiful
>>
