This message has been posted by: 612 right <i.hinners at REMOVE-THIS-TO-SENDicrf.icnet.uk>
Hi!
For collodial Blue use Coomassie G 250 and follow: Neuhoff et al,
Electrophoresis, 1988, 9, 255-262
They claim, you dont need to destain and its more sensitive, although I
never found that and returned to good old conventional
coomassie stain as Richard describes it.
Hope this helps, Ina
"Richard P. Grant" wrote:
> In article <109ec386.d1a7c612 at usw-ex0109-066.remarq.com>, David Coulson
> <d.coulsonNOd.SPAM at qub.ac.uk.invalid> wrote:
>> > I was wondering if anybody could help me with a "recipe" for
> > making up Collodial Blue (Comassie G-250) solution for
> > staining SDS-PAGE gels. Do you just make it up in water?,
> > and if so what sort of concentration?
>> Coomassie brilliant blue R250? There is something which sounds similar
> but doesn't work. Can't remember what it is, exactly.
>> Anyhoo: 0.1 % coomassie in 40% methanol, 10 % glacial acetic acid in
> water (e.g. 2g dye, 800 ml MeOH, 200 ml GAA, make up to 2 l). Store at
> 4C and can be reused several times. Stain for about 15 - 20 mins at RT.
> For destaining, I pour off the dye, then wash in dH20 (we have that on
> tap) twice, then wash in destain (same as above, minus the dye) with
> foam bungs to soak up the blue. This can also be re-used if you lid the
> container to reduce evaporation.
>> HTH, email me if you're stuck.
>> R
>> --
> Richard P. Grant MA DPhil
> Structural Studies Group, MRC-LMB
>http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html> Please reply to rpg 'at' mrc-lmb.cam.ac.uk
--
Ina Hinners
ICRF
Secretory Pathways Laboratory
44 Lincolns Inn Fields
WC2A 3PX London
UK
email: I.Hinners at icrf.icnet.uk
