hello,
I 'd like to purify a protein from saliva, but there were many problems
for it's viscosity.
I used whole saliva and removed cell and tissue debris by
centrifugation. But the supernatant still had strong viscosity.
Is it normal procedure to purify a protein from saliva with this strong
viscosity?
or are there any methods to eliminate viscosity without any harmful
effects on saliva protein?
If you have good references about protein purification in saliva, please
send me the information about that paper and so forth.
thanks in advance
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