On Sat, 4 Mar 2000 23:40:20, Anton Tutter <atutter at aim.salk.edu> wrote:
ð
ð > Ð We attach GST-pusion proteins to GSH-agarose, and need to keep the beads
ð > Ð for a long time for a frenzy of pull-downs we are doing. We find they
ð > Ð get bacterial contamination or lose activity after a month or so at
ð > Ð 4degC.
ð
ð
ð > Some beads -- I think including agarose -- are shipped in 20% EtOH. Unless
ð > this would kill the glutathione, you could store the beads at 4deg in 20%
ð > EtOH.
ð >
ð
ð i wouldn't do that if i were you!!! i think peter misunderstood the
ð questions -- the beads are to be stored long-term *after* the GST-fusion
ð proteins are bound to the beads.
Oops! I did misunderstand! Anton is quite right. In response to other
questions, the presence of glycerol helps prevent ice crystal formation
(that's why it's used for freezing bacterial cells).
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