Hi, Everyone!
I am purifying two gravity related esterase, enzyme are stable
at the present of low concentration of SDS and high resolution
of (NH4)2SO4, but the activity lost at the present of SDS after
the phenol sepharose column.
In column work,
starting and washing buffer : 50 mM Tris-Cl buffer containing
1.7 M (NH4)2SO4
eluted with gradient (NH4)2SO4 from 1.7 M to 0 M.
Where is the enzyme? How can I found the reson?
Please send me your idea.
With best regards.
Thanks a lot for your reply......
~~~~~~
chenggy at iris.sipp.ac.cn
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