Oddmund,
We've found that the 4-His antibody from Qiagen is very good for Western blot
staining, better in fact than there other antibodies in our hands.
We've been expressing 6-his tagged proteins in CHO cells with mixed degree of
success. One protein we made in E.coli worked very well (~10% of total
inclusion body protein) but was difficult to purify because it formed
multimers.
Hope this helps.
Roger
In article <38BD1B3E.B34CDD5D at biokjemi.uio.no>, Oddmund
=?iso-8859-1?Q?Nordg=E5rd?= <oddmundn at biokjemi.uio.no> wrote:
>Hello!
>>I`m planning to make a 6xHis tagged fusion protein. Afterwards I want to use
> the
>>tag as an epitope for immunofluorescence. Has anybody tried this? Which
> antibody
>>do you suggest?
>>Have anybody tried the 6xHis antibody from Clontech? Or the one from Amersham
>>Pharmacia Biotech? Dianova? Qiagen?
>>What is your experience with expression of His-tagged proteins in mammalian
> cell
>>systems?
>>Thank you very much for any help!
>>Oddmund
>>--
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>> Oddmund Nordgård
> Ph.D. student
> Institute of Biochemistry
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> Email: oddmundn at biokjemi.uio.no>> Private:
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