protein diffusing in SDS-PAGE

joe qqqwww at 21cn.com
Thu Jun 29 02:34:36 EST 2000

proteins I use 15% or 18% discontinuous SDS-PAGE gels,run at 150V until the dye front reaches the end of the gel (usually 50 minutes) and stained by G-250.I always find the band low than 14 MW is diffused,Anyone has experience in that and could give some suggestion?Thank you very much 


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