We're looking to express and purify a human protein of about 120 amino
acids in E. coli. Does anyone have any feelings, one way or the other,
about the following systems?
Clontech pPROTet.E
Promega PinPoint Xa
Stratagene pCAL
The Stratagene system looks pretty attractive, but we'd like to hear
from someone who's actually used one or all of these. Thanks for any
advice!!
Paul D. Guthrie
Urology Research Lab
Children's Hospital, Boston
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