Engineering a disulfide bond

Alan A. Dombkowski domski at umich.edu
Thu Jun 15 09:34:37 EST 2000


I may be of some help.  Email me.  Your email address seems
to be invalid.

On 9 May 2000, Larry wrote:

> My thesis work involves studying the protein-protein interactions
> between two enzymes.  One of the interaction domains is a short 69
> amino acid coiled-coil motif.  We've cloned and expressed this region
> in E. coli but want to engineer a disulfide bond to "close" off the
> loop (and make the motif more rigid).  I know that disulfide bond formation
> is subject to many variables (correct phi, psi angles, etc.).  Is
> there a computer or web-based program that will help me predict where
> I can insert two cysteines to create this disulfide bond?  Thanks.

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