Nuclear Extract

Paul S. Brookes. brookes at uab.edu
Tue Jun 6 08:22:20 EST 2000

By nuclear extracts do you mean just the DNA (in which case a simple 
precipitation method should work) or the whole nucleus including the 
membrane and any proteins there - which is a bit more tricky?

WRT markers, LDH is about as good a cytosolic marker as any - rock solid 
spectrophotometric rates and lots of kits available from Sigma e.t.c. if 
you can't be bothered to work up a method.  Mitochondrial markers are a bit 
more tricky - try citrate synthase (a matrix enzyme), as its easy to 
assay.  However your contamination might be from the mito' membrane 
fraction, so try monoamine oxidase (outer membrane) or cytochrome c oxidase 
(inner membrane).


Dr. Paul S. Brookes.            (brookes at uab.edu)
UAB Department of Pathology,   G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915     Fax (001) 205 934 1775

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