I would like to deglycosylate approx. 10 mg Protein for crystallisation.
The glycan is mannose-rich and Asn-N-linked.
Does anyone have experiences with native deglycosilation ?
(Most protocols, supplied with PNGase F or endo H are only for
analytical
purposes , max 100 µg for SDS-analysis under denaturating conditions)
ANY HINTS APPRECIATED, thanks in advance !
bye , Marco
--
Marco Bocola Dept. of pharmaz. Chemistry
AG Prof. Klebe
Tel: +49-6421-28-5071 Marbacher Weg 6
Fax: -8994 D-35032 Marburg
http://pc1664.pharmazie.uni-marburg.de/agklebe
e-mail:bocola at mailer.uni-marburg.de
:-( There are no problems, just solutions !? :-)
