Hi All,
I donot know if this is the right group to place this question ...!! but
..does anyone have any experience in solubilizing a protein ..found in
large amounts..in a Sf-9 cell line Pellet?? Molecular wt is around 60kD
..and..is estimated to be expressed at high conc...(150mg/lit) ...The
protein was harvested from large plates as well as a spinner flask 48 hrs
post infection.
Any help is higly appreciated
Thanks in advance.....
Jes
