TCA precipitation of proteins

Chris LaRosa clarosa at biocomp.unl.edu
Mon Jul 17 10:10:33 EST 2000

Suzanne Hermeling wrote:
> Hi
> I have a very brief question:
> When you perform a TCA precipitation of proteinsamples, do the proteins
> denature?
> Or can you "rebuild" them in a phospholipid membrane again?
> Suzanne

They are definitely denatured!... Thats why TCA ppt is recommended for
protease inactivation.

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