IUBio

In vivo chemical-crosslink??

Martin Offterdinger martin.offterdinger at akh-wien.ac.at
Wed Jul 5 09:10:37 EST 2000


Hi netters,
Is it possible to do in vivo chemical crosslinking, i.e. treat cells with a
crosslinker and isolate proteins afterwards in order to analyze protein
interactions via immunopreciptation and/or Western?
Which crosslinker (s) can be used for that purpose?
Which controls should be done?
Any input is highly appreciated!
Thank you!
martin







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