NEED PROTOCOL: PCRing genes from E.Coli

Chris LaRosa clarosa at biocomp.unl.edu
Mon Jul 3 13:52:15 EST 2000

> Can someone give a working protocol
> for PCRing a gene from E.Coli genome?
> Anton.

Step 1) Design good primers.

Step 2) Design good primers.

Step 3) Isolate genomic DNA according to myriad protocols available on
web with minimal search.  Also see Red cookbook or blue cookbook.

Step 4) Read the manual of your PCR machine and or Taq DNA polymerase
provider... Possible purchased from DnAMP Inc. or others.  Gibco BRL
gives a protocol with every tube of Taq polymerase, for instance.

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