Hi Luc,
N-terminal excision of the methionine is happens very often. It just depends
on the aminoacid following the methionine. I lost the reference but you will
find in the PubMed, I guess. If not, you can contact me.
Good Luck
Dietbert
Luc CAMOIN <camoin at cochin.inserm.fr> schrieb in im Newsbeitrag:
B4CDCBB6.1A45%camoin at cochin.inserm.fr...
> Dear Netters,
>> I purified a recombinant protein from E Coli. The N-terminal sequence and
> Mass spectrometry experiments show that the initial methionine is
eliminated
> in 90% of the purified protein.
>> Does anyone have any explanation about this phenomenon?
>>> Thanks in advance
>> Luc CAMOIN
>> --
> Luc CAMOIN
> Tel:+33 1 40 51 64 98
> Fax:+33 1 40 51 72 10
> Institut Cochin de Génétique Moléculaire / Groupe Chimie des Protéines
> Laboratoire d'Immuno-Pharmacologie Moleculaire / CNRS UPR 415
> 22 rue Méchain
> 75014 Paris France
> Internet: http://www.cochin.inserm.fr/upr415/UPR415E2.htm>>>
