Initial Methionine E Coli

Luc CAMOIN camoin at cochin.inserm.fr
Mon Feb 14 09:11:51 EST 2000

Dear Netters,

I purified a recombinant protein from E Coli. The N-terminal sequence and
Mass spectrometry experiments show that the initial methionine is eliminated
in 90% of the purified protein.

Does anyone have any explanation about this phenomenon?

Thanks in advance


Tel:+33 1 40 51 64 98
Fax:+33 1 40 51 72 10
Institut Cochin de Génétique Moléculaire / Groupe Chimie des Protéines
Laboratoire d'Immuno-Pharmacologie Moleculaire / CNRS UPR 415
22 rue Méchain
75014 Paris France 
Internet:    http://www.cochin.inserm.fr/upr415/UPR415E2.htm

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