Dear Netters,
I purified a recombinant protein from E Coli. The N-terminal sequence and
Mass spectrometry experiments show that the initial methionine is eliminated
in 90% of the purified protein.
Does anyone have any explanation about this phenomenon?
Thanks in advance
Luc CAMOIN
--
Luc CAMOIN
Tel:+33 1 40 51 64 98
Fax:+33 1 40 51 72 10
Institut Cochin de Génétique Moléculaire / Groupe Chimie des Protéines
Laboratoire d'Immuno-Pharmacologie Moleculaire / CNRS UPR 415
22 rue Méchain
75014 Paris France
Internet: http://www.cochin.inserm.fr/upr415/UPR415E2.htm
