stripping nitrocellulose membrane

Pavel Sova ps44 at columbia.edu
Mon Feb 7 10:16:15 EST 2000

On 4 Feb 2000, Sandra Pena wrote:

> Date: 4 Feb 2000 19:40:29 -0000
> From: Sandra Pena <spena at upracd.upr.clu.edu>
> Newsgroups: bionet.molbio.proteins
> Subject: stripping nitrocellulose membrane
> Can somebody give me a good protocol for stripping nitrocellulose
> membranes for immunoblots?
> Please send it to me at: sandra_vazquez at hotmail.com
> ---

How about this one:

Incubate your membranes in 0.2 M NaOH for 5 min.  Neutralize in TPBS or
TTBS (Tween - PBS etc.) and block with 5% milk in ditto. I think this is a
modification of protocol from Red Book, it works fine for me (might be a
little rough, though).

Pavel Sova, Ph.D.
New York
ps44 at columbia.edu

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