Frank Fuerst schrieb:
> Alexander wrote:
> >
> > bands on SDS-PAGE. I also noticed an increasing pressure of the column.
> > Summarized I got two results, either the proteins appeared in the void volume
> > or they disappeard. Thus my interpretation is that the proteins form aggregats
> > which either stick to the column or do not interact at all. It is confusing.
> > But I have no direct proof for the formation of aggregats. What else could
> > it be?
>> I also think that it is aggregation.
>> Have you tried working in the cold room?
>> Frank
Yes, I started to work at 4°C to minimize degradation (using buffer with PMSF,
Leupeptin, Pepstatin, Antipain), but it did not help.
Alexander
