Alexander wrote:
>> bands on SDS-PAGE. I also noticed an increasing pressure of the column.
> Summarized I got two results, either the proteins appeared in the void volume
> or they disappeard. Thus my interpretation is that the proteins form aggregats
> which either stick to the column or do not interact at all. It is confusing.
> But I have no direct proof for the formation of aggregats. What else could
> it be?
I also think that it is aggregation.
Have you tried working in the cold room?
Frank
--
> Weiterhin würde ich in "de.rec.mampf" vom Namen her nicht erwarten,
> daß die Gruppe auf vegetarische Gerichte beschränkt ist.
Das ist ein rekursives Acronym, genau wie GNU und heißt:
"Mampfe alles mit Pommes frites." [Stefan Fleiter in dang]