IUBio

extraction of protein from an ief gel

Anne Nicolas anne.nicolas at weizmann.ac.il
Thu Aug 3 07:59:46 EST 2000


I loaded my protein on an ief (isoelectro focusing) gel (pagplate gel
from pharmacia). I would like to recover the protein in its active form
from each band. Do you have any idea how to extract a protein from an
ief gel?
Thank you for your help
anne
 
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Anne NICOLAS
Weizmann Institute of Science
Structural Biology Department 
Kimmelman building
76100 Rehovot  Israel
tel +972-8-9343759/2128 fax  +972-8-9344159
email: anne.nicolas at weizmann.ac.il
home page:
http://www.weizmann.ac.il/Structural_Biology/Pages/Sussman/anne/index.html
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