Dr. Peter Gegenheimer wrote:
> On Tue, 1 Aug 2000 13:08:08, Byung-Hoon Kim <byung-hoon.kim at uni-tuebingen.de>
> wrote:
>> ð
> ð Hello everyone,
> ð
> ð I wonder if phenolic compounds im Arabidopsis can influence protein
> ð structure during the preparation of protein extract.
> ð Any comments will be greatly appreciated.
>> Phenolics bind tightly to many proteins (this is the basis for tanning
> leather!) and denature them. Phenolics can be sequestered by including PVP
> (polyvinylpyrrolidone) or PVPP (??) in the crude extract. There are several
> good methods papers on plant extracts that deal with this problem; an older
> one is my chapter in Meth Enz 182, 174-193 (1990).
>> o----------------------------------------------------------------------o
> | Dr. Peter Gegenheimer | Vox: 785-864-3939 FAX: 785-864-5321 |
> | Department of | PGegen at UKans.nospam.edu |
> | Molecular Biosciences | http://rnaworld.bio.ukans.edu/ |
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Another (cheaper) option is to add 50-100 mM bisulphite in your buffers to
prevent the phenolic compounds to start reacting.
---=== Bassie ===---
