On Tue, 1 Aug 2000 13:08:08, Byung-Hoon Kim <byung-hoon.kim at uni-tuebingen.de>
wrote:
ð
ð Hello everyone,
ð
ð I wonder if phenolic compounds im Arabidopsis can influence protein
ð structure during the preparation of protein extract.
ð Any comments will be greatly appreciated.
Phenolics bind tightly to many proteins (this is the basis for tanning
leather!) and denature them. Phenolics can be sequestered by including PVP
(polyvinylpyrrolidone) or PVPP (??) in the crude extract. There are several
good methods papers on plant extracts that deal with this problem; an older
one is my chapter in Meth Enz 182, 174-193 (1990).
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