Yes, they would, since the ab-ag complex is not covalent.
At 06:11 PM 4/25/00 -0700, Edward wrote:
>Hi, this might be a silly question, but I really need your help.
>I am using the product of immunoprecipitation for electrophoresis, that is,
>the samples are antibody-antigen complexes (is that right?).
>My question is that will there be a possiblity of degrading, or breaking of
>the ab-ag complexes, during the procedure of SDS-PAGE, which is a denaturing
>electrophoresis.
>Thanks in advance.
>Ed
>>>
Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu
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