Hi,
assuming that the biochemical characteristics of a given His-tagged
protein are very similar to the wild-type enzyme, is there any general
reason not to try and (attempt to) crystallize the His-tagged protein?
In other words, does an N-terminal His-tag (in general) interfere with
crystallization, or not? Are there any reviews out there that discuss
the issue?
Thanks in advance,
Uli.
