Is there anybody having experience on the purification of GST fusion
proteins?
Our lab doesn't have much experience and now there has been a serious
problem of degradation of the GST fusion protein. We have tried the
protease inhibitor from Sigma and Roche, and the whole purification
process was accomplished in a 4 C cold room. But the proteolysed
products are still there.
Any suggestions are highly appreciated. Thank you all for your
attention!
Eva Y. Chen
yi-hui.chen at mcmail.vanderbilt.edu
Jim Sutcliffe Lab
615-936-3627
Department of Molecular Physiology and Biophysics
Vanderbilt University School of Medicine
Nashville, TN 37232
USA
