Hello,
I am currently using two FLAG-tagged proteins to test for their interaction
properties. It was recently brought to my attention that FLAG tags can be
sticky -- I am wondering now whether the two FLAG tags may increase the
interaction of my two proteins. Does anyone out there have any experience
with this happening? I'm planning on running a control with a separate
epitope, but I'd still like to hear if anyone has any experience with two
FLAG peptides interacting.
Thanks
Neil
