Have you tried light scattering, or analytical centrifugation???
M.
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Miriam (Miri) Hirshberg e-mail: m.hirshberg at mole.bio.cam.ac.uk
Department of Biochemistry Tel : (+44) 01223 766018
University of Cambridge Tel : (+44) 01223 333600 (switch board)
80 Tennis Court Road Fax : (+44) 01223 766002
Old Addenbrooke's Site
Cambridge CB2 1GA, UK
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On Tue, 19 Oct 1999, Keri Freeland wrote:
> Hi,
>> I have a soluble but aggregated protein that I'm trying to prepare
> for NMR studies. I know it's soluble because the protein solution is
> clear, and I know it forms aggregates from running size exclusion
> columns.
>> I'm getting ready to test different buffer conditions (detergents,
> salt, pH), but I can't think of a good way to check my samples for
> monomeric protein. The time is more of an issue than amount of
> protein, since it expresses well.
>> Does anyone know a quick way to do this?
>> Thanks,
> Keri
>
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