Hi,
I have a soluble but aggregated protein that I'm trying to prepare
for NMR studies. I know it's soluble because the protein solution is
clear, and I know it forms aggregates from running size exclusion
columns.
I'm getting ready to test different buffer conditions (detergents,
salt, pH), but I can't think of a good way to check my samples for
monomeric protein. The time is more of an issue than amount of
protein, since it expresses well.
Does anyone know a quick way to do this?
Thanks,
Keri
