>Does anyone out there have info on stripping nitrocellulose membranes to
>reprobe with different antibodies? I recall that there was a procedure with
>the chemilluminescent methods, but I forget the details.
try this method:
wash blot 4 x with PBS (5 min/wash)
incubate for 30 min at 50 deg. C in:
62.5mM Tris-HCl pH 6.8
wash 6 x 5 min in PBS.
block as usual.