IUBio

Need help:insoluble to soluble

Jared Quentin LeMaster lemaster.34 at osu.edu
Mon Oct 4 14:15:35 EST 1999


Hi:
   I expressed foreign protein in E.coli to the level of about 40% total
protein after IPTG induction. But it is insoluble. Does anyone have any
experiences that could make it soluble without loss of function or
expressed as a soluble protein in E.coli?  Pls send your suggestion to
wang.508 at osu.edu. Thanks a lot.



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