This is a multi-part message in MIME format.
--Boundary_(ID_aFjp0A6VPEaopwbyV6r8mA)
Content-type: text/plain; charset=iso-8859-1
Content-transfer-encoding: 7BIT
Hi everybody. I have a problem: I am trying to set up a typical NADH coupled assay to measure Pyrvate formation (using LDHase). One of the proteins of my system is a transmembrane protein, and I have to use it as a membrane extract. The problem is that in the membrane there seems to exist a second protein which interferes with the coupled assay, because it consumes NADH by a different and unknown pathway (I have not found yet the substrate being reduced). The solution would be very simple if I can find any general irreversible inhibitor for proteins which use NADH.
Does anybody know any such inhibitor??
Thank you very much for any information.
--Boundary_(ID_aFjp0A6VPEaopwbyV6r8mA)
Content-type: text/html; charset=iso-8859-1
Content-transfer-encoding: 7BIT
<!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN">
<HTML>
<HEAD>
<META content=text/html;charset=iso-8859-1 http-equiv=Content-Type>
<META content='"MSHTML 4.72.3110.7"' name=GENERATOR>
</HEAD>
<BODY bgColor=#ffffff>
<DIV><FONT color=#000000 face=Arial size=2> <FONT
color=#000000>Hi everybody. I have a problem: I am trying to set up
a typical NADH coupled assay to measure Pyrvate formation (using LDHase). One of
the proteins of my system is a transmembrane protein, and I have to use it as a
membrane extract. The problem is that in the membrane there seems to exist a
second protein which interferes with the coupled assay, because it consumes NADH
by a different and unknown pathway (I have not found yet the substrate being
reduced). The solution would be very simple if I can find any general
irreversible inhibitor for proteins which use NADH.</FONT></FONT></DIV>
<DIV><FONT color=#000000 face=Arial size=2><FONT
color=#000000> Does anybody know any such
inhibitor??</FONT></FONT></DIV>
<DIV><FONT color=#000000 face=Arial size=2><FONT
color=#000000></FONT></FONT> </DIV>
<DIV><FONT color=#000000 face=Arial size=2><FONT
color=#000000> Thank you very much for any
information.</FONT></FONT></DIV>
<DIV><FONT color=#000000 face=Arial size=2><FONT
color=#000000></FONT></FONT> </DIV></BODY></HTML>
--Boundary_(ID_aFjp0A6VPEaopwbyV6r8mA)--