On Thu, 25 Nov 1999 14:01:47 GMT, Serge Champetier
<aca611 at agora.ulaval.ca> wrote:
>I am seeking a simple system for overexpression of a 50 amino acid
>protein. High yield (at least 10 mg) and good purity of the protein are
>needed, but no post-translational modification is required. Is the GST
>fusion protein system in E.coli a good choice?
>Serge Champetier, M.Sc.
>Quebec City, Canada.
>
I think you should consider the pET system from NOVAGEN
There are a lot of different vectors available.
The expression is IPTG inducible (high amount of protein)
You have the advantage of an His-Tag (easy purification via Ni-column)
which is removeable.
For that System you also need a special E.coli strain (BL21-DE3) which
is also available from NOVAGEN.
Cheers
Mark
