IUBio

copper binding column

ted michelin at ohsu.edu
Wed Nov 24 03:08:01 EST 1999


Any of the so called metal chelate columns used for His tagged protein
purifications will bind Cu, Pharmacia has a nice chelating column and they
even have data on the Cu binding activity in the catalog, I think its all
Cu2+ so if you have a different valency you may need to fond another
resin. Good luck

Ted Michelini
OHSU
michelin at ohsu.edu

arjen wrote:

> I 'm currently investigating curative effects of certain plant extracts
> on copper intoxicatin in snail embryo's. Results are great, but  in
> order to investigate wether this plant extract is merely a copper
> chelator i 'm looking for a column wich will bind copperbinding
> substances (not just proteins). The idea is to lead the extract over
> this column and test or the substance is still active.
> Does anyone have a suggestion? Thanx.





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