That's why we love proteins! Different proteins can have very different
properties (not just "add 1/10th volume NaOAc, 2 volumes EtOH, leave at -20C
overnight, spin 10 minutes....")
I once worked with a protein which stained a faint purple with CBB R-250,
was soluble in 10% TCA, and totally resistant to proteinase K. My wife at
the same time was working with proteins which would not stain with silver
(even with microgram quantities in a band) until the silverstain process was
repeated a second time.
So just work with the white spot!
Thorsten Burmester <thorsten at erfurt.thur.de> wrote in message
news:38331EDB.7CDACC51 at erfurt.thur.de...
> After SDS-PAGE and Western transfer to PVDF I stained the proteins with
> 0.1% Coomassie Blue in H20 dd. To my surprise the band of interest was
> _not_ stained but remained white on a light blue background. Other
> proteins were stained well as expected. Any explanation?
>> TIA,
>> Thorsten
>> --
> Thorsten Burmester
>thorsten at erfurt.thur.de
