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Native PAGE woth low pH

Bernard Murray, PhD spam at 127.0.0.1
Fri Nov 19 16:55:55 EST 1999


In article <812to1$76o$1 at post.student.mu-luebeck.de>, "Lars Komorowski"
<larskomo at biochem.mu-luebeck.de> wrote:

> Can someone provide me with a protocol for a native PAGE with pH lower than
> 6.5 ?

Depending on the pI of your protein of interest you may need to
reverse the polarity of the gel (run from + to -).

   Bernard

-- 
Bernard P. Murray, PhD
bpmurray at cgl . ucsf . edu
Department of Cellular & Molecular Pharmacology, UCSF




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