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Coomassie staining of proteins on PVDF

Thorsten Burmester thorsten at erfurt.thur.de
Wed Nov 17 16:32:11 EST 1999


After SDS-PAGE and Western transfer to PVDF I stained the proteins with
0.1% Coomassie Blue in H20 dd. To my surprise the band of interest was
_not_ stained but remained white on a light blue background. Other
proteins were stained well as expected. Any explanation?  

TIA,

Thorsten

--
Thorsten Burmester
thorsten at erfurt.thur.de




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