Details...are you using cell extracts? a cell free system? Media?
There are a bunch of substrates that you can use but a few are unstable or
are teratogenic. Fluorescent substrates are by far the best to use in terms
of sensitivity. H2O2 is suprisingly a stable molecule.
If you are looking for in vivo assays, James McNamara over there at Duke
(Epilepsy centre, I think) has done a few of these.
William Tschantz wrote:
> I am looking for a sensitive assay for H2O2 (hydrogen peroxide). I think
> the enzyme that i am working on may generate it. I am thinking of
> possible using a peroxidase linked assay. Has anyone done this before?
> Any help would be appreciated!
--
---
Austin P. So (Hae Jin)
I.I.S.G.P.
Biotechnology Laboratory
University of British Columbia
E-mail: haejin at netinfo.ubc.cahttp://www.interchange.ubc.ca/haejin/index.html (under construction)
