I recall doing this with luminol/hemoglobin at some time in the distant
past. A chemiluminescence is generated with an oxidant such as H2O2.
It's quantitated with a luminometer or fluorimeter. Sorry, but no
references.
William Tschantz wrote:
>> Hi
>> I am looking for a sensitive assay for H2O2 (hydrogen peroxide). I think
> the enzyme that i am working on may generate it. I am thinking of
> possible using a peroxidase linked assay. Has anyone done this before?
> Any help would be appreciated!
>> Thanks in advance
>> Bill
>> --
> Bill Tschantz, Ph.D.
> Duke University Medical Center
> Dept of Pharmacology and Cancer Biology
> C307 Levine Science Research Center (LSRC)
