In article <383175A8.3865DDA3 at uni-tuebingen.de>, Byung-Hoon Kim
<byung-hoon.kim at uni-tuebingen.de> wrote:
> Hi, all
>> Can proteinase inhibitors (Aprotinin, Leupeptin and Pepstatin) penetrate
> the cell membrane?
>>> Byung-Hoon Kim
No, leupeptin can't, not sure about the others but I doubt it. They are
taken up by endocytosis and act on lysosomal proteases AFAIK. However,
there are some protease inhibitors which reputedly are membrane permeant.
The one I know is E64-d, a dervative of the cysteine protease inhibitor E64
(which is membrane impermeant). E64-d will inhibit cytoplasmic calpain I
believe.
--
Fergus Doherty,
School of Biomedical Sciences,
Nottingham University,
Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)
