Dear collegues,
We are trying to look at the creatine kinase enzyme activitiy of
different tissues of channel catfish. We run starch gels and
histochemically looked at the enzyme activities by using postcoupling
technique. We used acidic and basic buffers and run total proteins. We got
high enzyme activity at cardiac, skeletal and smooth (stomach) muscles.
However, in both acidic and basic buffer conditions, the stomach bands
showed up at the cathodal side. Does anybody have any clue about cathodal
CK enzymes? Thanks.
Huseyin Kucuktas
