In article <pxpst2+-0611991043130001 at pelli.pathology.pitt.edu>,
pxpst2+ at pitt.edu (Peter) wrote:
> In article <3821AEEE.9EFC3D58 at sheffield.ac.uk>, Lee Hunt
> <L.hunt at sheffield.ac.uk> wrote:
[snip]
> Always remember that PAGE does not seperate purely by mass/size.
> Seperation is by the charge to mass (m/z). Alter this and and you
> will have abherent migration.
This is why we include SDS in our PAGEs. If it were so that proteins
bound a number of SDS molecules proportinal to their mass and that this
number was so large that the proteins own charge would be negligable
compared to the charges that SDS bring, then all proteins in SDS would
have approximately the same m/z. The idea is then, that you separate on
m (or rather volume or some other structure parameter) using the sieving
effect of the PA gel.
Kresten
>> Regards,
> Peter Pediaditakis
>
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