IUBio

Need help:insoluble to soluble

Hein hein28NOheSPAM at yahoo.com.invalid
Mon Nov 8 23:51:02 EST 1999


Try purifying the inclusion body, solubilizing it in 8M urea, and
slowly refolding it. Works wonders for many proteins; esp. if you have
an affinity column to help select active refolded proteins.




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