Help:what had happened in western blot?

[nshh at 163.NET]

Hi everyone,

After the total protein of E.coli was separated by PAGE, and transferred to 
PDGF (and NC) membrance,a special antibody was used to do western blot as normal,but all the E.coli protein disappeared on the membrance, who can tell me what had happened? It will help me a lot.

thanks 

David Neer

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