Has anybody used Streptolysin O for permeabilizing some cells? I'm trying
to permeabilize mast cells with SLO. And an unexpected
problem came up. I don't know
how to store the reagent in stable state. I bought it from Sigma and
dissolved with water, aliquoted, and froze it. And just before using
it, I treated it with 20mM of DTT to activate it because SLO is readily
oxidized in solution, which is inactive . On the first day I got
my cells permeabilized by SLO treatment. But from the next day, I have
never
got the same result even though I used ten times higher concentration of
SLO. Sigma says SLO solution should be made fresh , but it's almost
impossible because one bottle has only 900ug. This is frustrating. Does
anybody have an answer to this problem? Please let me know ASAP, please.
Thanks in advance.
Jeoung-Sook Shin
