Dear all,
I have recently had problems with my FPLC gel filtrations using yeast
extracts. In my dispair, I added every single available protease
inhibitor to my extract. Now I have a beautiful result but the protein
comes out with approximately double the size and also runs according to
doubled size on the gel. So, either I have modified my protein or stably
cross-linked it with itself or another protein or ...
The inhibitor I suspect having caused this is iodoacetamide. Can anyone
tell me what the effects of iodoacetamide on proteins are?
Thank you very much for your help.
Claus
Please reply to Claus.Schwechheimer at yale.edu
