Michael Wing-Yan CHAN <bomchan at ust.hk> wrote:
> I am expressing a histidine-tagged protein in E.coli
> and I've found that, the protein is secreted out of the the bacteria.
> Do u have any suggestion of how to purify it.
Does the protein in the medium bind to Ni agarose? If yes, you're all
set.
--Cornelius.
--
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP4 */
/* "Science is the game we play with God to find out what His rules are." */
