Hi Netters
I have a question here......
Can anyone tell me...if it's possible .... PRACTICALLY....... to get
proteins into solution after being transferred on membrane (NC,
PVDF....whatever..)? Say....to increase protein purity and
concentration.
I never did that...but ...who knows..maybe you or your friends did?
Say...to chop membrane with certain protein band, add
SDS/tween/urea/TX....detergents in buffer....boil....incubate etc.etc.?
Any suggestions would be highly appreciated.
Regards
Levan Darjania
Austin, TX